Satoshi TSUNEDA, Associate Professor

(Chemical Engineering)

(b. 1965), B. Engr. (1989, Tokyo), M. Engr. (1991, Tokyo), Ph.D. (1994, Tokyo), Res. Fellow(1994-1995, Chiba), Post Doc. (1995-1996, RIKEN), Assist. Prof. (1996, Waseda).
The Award of the Society of Chemical Engineer for Young Researcher (1994).


keywords

Chemical Engineering / Transport Phenomena / Functional Material / Bioseparation / Graft Polymerization


Room 60-113
3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan

Tel: +81-3-5286-3210

Fax: +81-3-3209-3680

E-mail:stsuneda@waseda.jp


Research Interests

Interaction between biomolecules and solid surface has been applied to many technical fields such as bioreactor, bioseparation and biosensor technologies. We are aiming to design biointerface where microorganisms make their communities, and apply it to biological wastewater treatment processes. In view of engineering, it is greatly advantageous for wastewater treatment to immobilize microorganisms onto surfaces of small carrier particles and fluidize them in the reactor. A "biofilm", which is a thick layer made up of microorganism clusters, can be obtained by elucidating and controlling an interaction between a microorganism cell and solid surface. We are trying to monitor microbial ecology of biofilms using molecular probes in order to understand their structures and functions, which is expected to enable the successful application for efficient wastewater treatments. Following topics are now investigated.

[Basic and practical study on biofilm]
- Biofilm formation on controlled surface
- Extracellular polymeric substances for biofilm
- Microbial ecology detected by molecular tools
- Mathematical analysis by diffusion-reaction model
- Granule process using aerobic upflow fluidized bed

[Nutrient removal from wastewater]
- Denitrifying phosphorus accumulating organisms
- Autotrophic nitrogen removal in single reactor
- Nitrogen removal from metallurgic wastewater
- Membrane-aerated biofilm reactor

[Treatment of biorefractory wastewater]
- Metal removal using sulfate reducing bioreactor
- Oxidation of organic compounds by UV/H2O2

[Others]
- Isolation and analysis of nonculturable bacteria
- Real-time monitoring of bacterial activity by mRNA
- Novel gene screening by microarray technique
- Wastewater treatment approaching to zero-emission


Representative Publications

1. "Characterization of Nitrifying Granules Produced in an Aerobic Upflow Fluidized Bed Reactor", Water Res., 37, 4965-4973 (2003).

2. "In Situ PCR for Visualizing of Distribution of a Functional Gene amoA in a Biofilm Regardless of Activity", J. Biotechnol., 105, 33-40 (2003).

3. "Influence of the Growth Phase on Bacterial Cell Electrokinetic Characteristics Examined by Soft Particle Electrophoresis Theory", J. Colloid Interface Sci., 264, 565-568 (2003).

4. "Adsorption Effect on Dynamic Response of Biochemical Reaction in a Biofilm Reactor for Wastewater Treatment", Eng. Life Sci., 3, 371-375 (2003).

5. "Extracellular Polymeric Substances Responsible for Bacterial Adhesion onto Solid Surface", FEMS Microbiol. Lett., 223, 287-292 (2003).

6. "Influence of Extracellular Polymers on Electrokinetic Properties of Heterotrophic Bacterial Cells Examined by Soft Particle Electrophoresis Theory", Colloids and Surfaces B: Biointerfaces, 29, 181-188 (2003).

7. "Quinone Profiles Reflecting Population Dynamics of Denitrifying Phosphate-Accumulating Organisms", Microb. Environ., 18, 69-73 (2003).

8. "Nitrogen Removal Characteristics and Biofilm Analysis of a Membrane-Aerated Biofilm Reactor Applicable to High-Strength Nitrogenous Wastewater Treatment", J. Biosci. Bioeng., 95, 170-178 (2003).

9. "Characterization of Microbial Community in Nitrogen Removal Process of Metallurgic Wastewater by PCR-DGGE", Water Sci. Technol., 46[11-12], 93-98 (2003).

10. "Dynamic Modeling and Simulation of a Three-Phase Fluidized Bed Batch Process for Wastewater Treatment", Process Biochem., 38, 599-604 (2002).

11. "Transformation of Phosphorus and Relevant Intracellular Compounds by a Phosphorus-Accumulating Enrichment Culture in the Presence of Both the Electron Acceptor and Electron Donor", Biotechnol. Bioeng., 79, 83-93 (2002).

12. "Real-Time Monitoring of Ammonia-Oxidizing Activity in a Nitrifying Biofilm by amoA mRNA Analysis", Water Sci. Technol., 46[1-2], 439-442 (2002).

13. "Simultaneous Nitrification and Denitrification by Controlling Vertical and Horizontal Microenvironment in a Membrane-Aerated Biofilm Reactor", J. Biotechnol., 100, 23-32 (2002).

14. "Three-Dimensional Immobilization of Bacterial Cells with Fibrous Network and its Application for a High-Rate Fixed-Bed Nitrifying Bioreactor", J. Chem. Eng. Jpn., 35, 68-75 (2002).

15. "Selection and Dominance Mechanisms of Denitrifying Phosphate-Accumulating Organisms Cultivated in Biological Phosphorus Removal Process"CBiotechnol. Lett., 23, 2005-2008 (2002).

16. "Direct Detection by in situ PCR of amoA Gene in Biofilm Resulting from a Nitrogen Removal Process", Appl. Environ. Microbiol., 67, 5261-5266 (2001).

17. "Kinetics of Tetrachloroethylene (PCE) Gas Degradation and Byproducts Formation during UV/H2O2 Treatment in UV-Bubble Column Reactor", Chem. Eng. Sci., 56, 6199-6207 (2001).


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